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Emily Sprague

Jackson, MI, USA

Key Skills
GIS and other software:
• ArcGIS Pro
• ArcGIS Desktop
• ERDAS Imagine
• QGIS
• Python and the ArcPy library
• R and the ggplot2 package
• SQL
• Geodatabase management
• Visual Studio Code
• GitHub
• RegEx
• Google Sheets and MS Excel
Laboratory:
• DNA extraction
• PCR and qPCR
• Microscopy
• Plant care
• Spectrophotometry
• Media preparation
• Bacteria culturing
• Mammalian cell culturing
Other:
• Scientific writing and editing
• Project management
• Troubleshooting
• Field work
• Data visualization
• Statistical analysis

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Professional Experience
Research assistant: Eastern Michigan University, Ypsilanti, MI February 2020 – present
• Caring for several thousand individually grown prairie legumes under sterile conditions, including inoculation with various soil samples and isolated rhizobia strains, biomass collection, and nodule counting
• Collecting over two thousand strains of rhizobia and associated bacteria from legume nodules and isolating and storing them for future use
• Assisting students with prairie field experiment set-up
• DNA extraction and PCR of isolated strains for identification by 16S sequencing
• Organization of large dataset mentioned above in set of large Google Sheets spreadsheets
• Automation of cryovial assignment to freezer box and box coordinates using Google Sheets, SQL, and RegEx
• Management and training of multiple graduate and undergraduate students

Freelance editor: Cactus Communications, freelance remote October 2019 – February 2020
• Edited scientific manuscripts, primarily written by non-native English writers, for grammar and clarity
• Formatted clients’ manuscripts for submission to scientific journals
• Provided lists of rules and tips to clients to assist future English-language writing

Biology expert: Study.com, freelance remote September 2019 – February 2020
• Concisely answered student questions about biology and related topics

Research technologist: University of Chicago, Chicago, IL September 2018 – November 2019
• Cultured 3D human colon organoids from fresh biopsies
• Tested the effects of various compounds on organoid chromatin accessibility using PCR and ATAC-seq
• Analyzed human gut microbiome 16S sequence data using R and QIIME
• Extracted accessible chromatin and performed qPCR on colon organoids
• Extracted DNA from human blood
• Trained other technicians, postdoctoral candidates, and undergraduate interns

Teaching assistant: Western Michigan University, Kalamazoo, MI September 2015 – April 2018
• Taught two sections of undergraduate students per semester for various biology courses
• Prepared lab lectures and homework

Lab assistant: Central Michigan University, Mt. Pleasant, MI August 2008 – May 2012
• Prepared cultures and media for undergraduate microbiology course
• Organized and cleaned the prep room and course lab

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Education
GIS Professional Certificate (expected April 2022)
Eastern Michigan University, Ypsilanti, MI

M.S. Biological Sciences: Microbial Ecology, 2018
Western Michigan University, Kalamazoo, MI

B.S. Biology: Microscopy concentration (major), Chemistry (minor), Magna Cum Laude, 2012
Central Michigan University, Mt. Pleasant, MI

Emily Sprague

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